Application of microcolumn gel technology in detection of neonatal hemolytic disease

VernacularTitle:微柱凝胶技术在新生儿溶血病检测中的应用
Author: Jingjing WU ; Wanbo YUAN ; Sisi XIE ; Xiulin XIAO
Publication Type:Journal Article
Keywords: Microcolumn gel technology; Hemolytic disease of newborn; Direct antiglobulin test; antibody release test; free antibody test
From: International Journal of Laboratory Medicine 2015;(7):872-874
CountryChina
Language:Chinese
Abstract: Objective To investigate the application value of microcolumn gel technology in screening hemolytic disease of the newborns(HDN) .Methods The direct antiglobulin test(DAT) ,antibody release test and free antibody test were performed in 212 cases of suspected HDN in our hospital by using microcolumn gel assay .Results In 212 cases of suspected HDN ,50 cases(23 .6% ) were diagnosed as HDN ,including 45 cases (21 .2% ) of ABO‐HDN and 5 cases (2 .4% ) of Rh‐HDN .In 45 cases of ABO‐HDN ,23 cases (36 .5% ) were A blood type and 22 cases (28 .2% ) were B blood type .The sensitivity of antibody release test ,DAT and free antibody test was 100% ,28% and 92% respectively .Conclusion The microcolumn gel technology can detect HDN fastly and accu‐rately ,with the advantages of simple operation ,less sample consumption ,high sensitivity and specificity ,which can provide reliable basis for HDN diagnosis and is worth popularizing and applying in clinic .