Fasudil protects from hepatic ischemia reperfusion injury in rats model of obstructive jaundice
10.3760/cma.j.issn.1007-8118.2015.01.012
- VernacularTitle:法舒地尔对阻塞性黄疸大鼠肝缺血再灌注损伤的影响
- Author:
Genglong ZHU
;
Chaonong CAI
;
Yingbin JIA
;
Xiaopeng HONG
;
Baimeng ZHANG
- Publication Type:Journal Article
- Keywords:
Fasudil;
Obstructive jaundice;
Ischemia reperfusion injury
- From:
Chinese Journal of Hepatobiliary Surgery
2015;21(1):44-48
- CountryChina
- Language:Chinese
-
Abstract:
Objetive To investigate the effect of Fasudil on the hepatic ischemia repeffusion injury on rats of obstructive jaundice.Methods 160 mature SD rats were randomly assigned into 2 large groups,namely experimental group (Group A) and control group (Group B).While each large group was subdivided into 4 subgroups,including sham operation subgroups(Group A1 and B1),obstructive jaundice model subgroups(Group A2 and B2),ischemia reperfusion model subgroups (Group A3 and B3)and obstructive jaundice plus ischemia reperfusion model subgroups (Group A4 and B4).In Group A2 and B2,obstructive jaundice models were made on rats by double ligating and cutting the common bile ducts.In Group A3 and B3,the ischemia reperfusion injury models were built by blocking the hepatic portal vessel for 30 minutes before reperfusion.In Group A4 and B4,the ischemia reperfusion injury models were built in 1 week after building the obstructive jaundice models.For Group A,10 mg/kg fasudil was intraperitoneally injected at 30 minutes before ischemia.For Group B,equal amount of physiological saline was injected.Samples were gained on zero hour,1 hour,2 hour and 6 hour post reperfusion.The serum indexes for liver function and serum Endothelin 1 level were tested and analyzed through SPSS.Pathological changes of liver were viewed correspondingly.Results Compared to control groups (Group Bs),experimental groups (Groups As) have better liver function,lower Endothelin 1 level (P ＜ 0.05),as well as less severe microscopic histological damage.Conclusion Fasudil can effectively protect rats of obstructive jaundice from hepatic ischemia reperfusion injury.
