Determination of Enantiomer in Rivaroxaban by HPLC
- VernacularTitle:HPLC法测定利伐沙班中对映异构体的含量
- Author:
Qianru ZHANG
;
Qi ZHANG
;
Xiaofeng GUO
;
Wenmin GUO
- Publication Type:Journal Article
- Keywords:
Rivaroxaban;
Enantiomer;
HPLC;
Determination
- From:
China Pharmacist
2014;(10):1629-1631
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the determination of enantiomer in rivaroxaban by HPLC. Methods:A Chiralpak IA column (250 mm × 4. 6 mm, 5 μm) was used with the mobile phase of 100% methanol. The flow rate was 1. 0 ml·min-1 . The detection wavelength was set at 250 nm and the column temperature was 40℃. Results:The resolution of rivaroxaban and the enanti-omer was above 2. 0. The linear range of them was 0. 5-1 000. 00 μg·ml-1(r=0. 999 9). The detection limit of the enantiomer was 0. 17ng and the average recovery was 102. 9% with RSD of 1. 54%(n=6). Conclusion:The method is accurate and rapid, and suit-able for the determination of the enantiomer in rivaroxaban.
