The time conserving blood sample at room temperature and the confidence of quantifying % TCD4, % TCD8, % lympho, WBC, MFI CD4, MFI CD8
- Author:
Tran Khiem Hung
- Publication Type:Journal Article
- Keywords:
Conserving blood
- MeSH:
Blood;
cells;
Temperature
- From:Journal Ho Chi Minh Medical
2004;8(3):143-147
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
The research aimed to establish the maximum time elapsed between collection and analysis of samples. The blood samples were stored at temperature varying from 200C to 280C. Studied on 32 subjects, all samples were marked by the monoclonal antibodies. Each sample had been analyzed 4 times: within 6 hours, 24 hours, 48 hours and 72 hours after drawing. Epi-info 2000 had been used for statistical analysis the results. Results showed that: the maximum elapse of time for the CD4 was 72 hours, for the CD8 was 24 hours and for the lympho and the WBC's as little time as possible, because the results were very different every second time
