Determination of AsperosaponinⅥ in Dieda Cuyu Tablets by HPLC
- VernacularTitle:HPLC法测定跌打促愈片中川续断皂苷Ⅵ的含量
- Author:
Pinglan ZHOU
;
Hui YU
;
Mengliang JIANG
;
Chunlin ZHANG
;
Xiaofei JIN
;
Hualan WANG
;
Xiaojuan YIN
- Publication Type:Journal Article
- Keywords:
DiedaCuyutablets;
Determination;
HPLC;
AsperosaponinⅥ
- From:
China Pharmacist
2014;(10):1770-1772
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the determination of asperosaponinⅥin Dieda Cuyu tablets by HPLC. Methods:A Hypersil C18(250 mm ×4.6 mm,5 μm)column was used. The mobile phase was acetonitrile-water(30∶70) with a flow rate of 1.0 ml·min-1 . The detection wavelength was 212 nm, the column temperature was room temperature,and the injection volume was 10μl. Results:AsperosaponinⅥ showed a good linear relationship within the range of 0. 04-0. 32 μg(r=0. 999 6). The average recovery was 97. 84%(RSD=1. 70%, n=6). Conclusion:The method is simple,accurate and reproducible, which can be used in the deter-mination of asperosaponinⅥ in Dieda Cuyu tablets.
