Determination of Thymol in Thymol Alcoholic Solutions by HPLC
- VernacularTitle:HPLC法测定麝香草酚醇溶液中麝香草酚的含量
- Author:
Chonghui HE
;
Lei GAO
;
Yanhua JIA
;
Xiaoqing WANG
;
Guiyang LIU
- Publication Type:Journal Article
- Keywords:
Thymol;
Thymolalcoholicsolution;
HPLC;
Contentdetermination
- From:
China Pharmacist
2014;(10):1782-1783
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination of thymol in thymol alcoholic solutions. Methods: An Agilent Eclipse XDB-C18(250 mm ×4.6 mm,5μm) column was used with the mobile phase of methanol-water (65∶35), the flow rate was 1. 0 ml·min-1 . the detection wavelength was 275nm, the injection volume was 10μl, and the column tenperature was 25℃. Re-sults:A good linear correlation of thymol was observed within the range of 60-160 μg·ml-1(r=0. 999 5). The average recovery was 101. 59% with RSD of 1. 39%(n=9). Conclusion:The method is quick, simple and accurate, which can be used in the determina-tion of thymol alcoholic solutions with good selectivity and sensitivity.
