Determination of Chloramphenicol Vaginal Tablets by HPLC
- VernacularTitle:HPLC法测定氯霉素阴道片中氯霉素的含量
- Author:
Zhengyuan SHI
;
Weixin ZENG
;
Lulu SUN
- Publication Type:Journal Article
- Keywords:
HPLC;
Chloramphenicol;
Vaginaltablets;
Determination
- From:
China Pharmacist
2014;(10):1788-1789
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination of chloramphenicol in chloramphenicol vaginal tablets. Methods:An Agilent ZORBAX SB-C18 column (150 mm × 4. 6 mm,5 μm)was used. The mobile phase was composed of 0. 1% sodi-um 1-heptanesulfonate solution-methanol (68∶32),the flow rate was 1. 0 ml·min-1, the UV detection wavelength was 277 nm,the column temperature was 35℃,and the injection volume was 10 μl. Results:The linear range of chloramphenicol was 25. 6-512. 0 μg ·ml-1(r=0. 999 9). The mean recovery was 99. 4%, and RSD was 0. 8%(n=9). Conclusion:The method is simple,accurate and reproducible,and can be used in the determination of chloramphenicol vaginal tablets.
