Determination of Stilbence Glucoside in Polygonum Multiflorum by HPLC
- VernacularTitle:HPLC法测定何首乌中二苯乙烯苷的含量
- Author:
Ting LAN
;
Baolong WANG
;
Minghua LI
;
Muqiong LI
;
Wei HE
- Publication Type:Journal Article
- Keywords:
Stilbeneglucoside;
Polygonummultiflorum;
HPLC;
Determination
- From:
China Pharmacist
2014;(10):1774-1775
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the content of stilbene glucoside in polygonum multiflorum by HPLC. Methods: An Agilent Eclipse Plus C18(100 mm ×4.6 mm,3.5 μm)column was adopted with acetonitrile-water(18∶82) as the mobile phase at a flow rate of 1. 0 ml·min-1 . The detection wavelength was set at 320nm and the injection volume was 8 μl. Results:The linear range of stilbene glucoside was 38-237. 5 μg·ml-1(r=0. 999 4)with the average recovery of 98. 32%(RSD=1. 59%). Conclusion: The method is simple, sensitive and specific in the content determination of stilbene glucoside in polygonum multiflorum.
