Matrix-assisted laser desorption ionization-time of flight mass spectrometry for rapid identification of clinical isolates
- VernacularTitle:基质辅助激光解析电离飞行时间质谱仪在快速鉴定临床酵母菌中的应用
- Author:
Haiyan XI
;
Ying WANG
;
Mei HUANG
;
Linghui LI
;
Ming FAN
;
Haifeng SHAO
;
Weiping WANG
;
Xiaojun LI
- Publication Type:Journal Article
- Keywords:
Matrix-assisted laser desorption ionization-time of flight mass spectrometry;
Clinical yeast;
Rapid identification
- From:
Journal of Medical Postgraduates
2015;(4):416-419
- CountryChina
- Language:Chinese
-
Abstract:
Objective Laboratory tests of yeast, with their disadvantages of long identification, complicated operation, and low positive rate, cannot meet the needsof rapid diagnosis and timely treatment.This studyinvestigated the feasibility ofapplying matrix-assisted laser desorption ionization-time of flight mass spectrometry ( MALDI-TOF-MS) in rapid identification and clinical isolationof yeast strains. Methods A total of 120 clinical non-repetitive isolates were collected from clinical culture samples and identified by MALDI-TOF-MS and VITEK 2-Compact/API, respectively.The discordant results were resolved by ITS gene sequencing. Results Of the 120 yeast isolates analyzed, 117 (97.5%) were correctly identified at the species level by MALDI-TOF-MS, 1(0.8%) misidenti-fied, and 2 ( 1.7%) unidentified.ITS gene sequencing of the 3 isolates showed the coincidence rate to be 0( 0/3) for MALDI-TOF-MS and Vitek 2-Compact/API. Conclusion MALDI-TOF-MScan be used as a rapid, accurate, and inexpensive tool for the identification of clinical yeast strains.
