Metabolic Effects of Nonsteroidal Anti-Inflammatory Drugs on Chondrocytes in Human Osteoarthritis
10.3969/j.issn.0253-9896.2013.09.006
- VernacularTitle:非甾体抗炎药对人骨关节炎软骨细胞生长代谢的影响
- Author:
Luling LI
;
Yi ZHENG
- Publication Type:Journal Article
- Keywords:
anti-inflammatory agents,non-steroidal;
osteoarthritis;
chondrocytes;
cell proliferation;
metabolism
- From:
Tianjin Medical Journal
2013;(9):867-870
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of nonsteroidal anti-inflammatory drugs (NSAIDs) on the proliferation of chondrocytes and metabolism of human osteoarthritis (OA) in short-term (48 hours). Methods The human knee OA chondrocytes were cultured in the presence or absence of NSAIDs (celecoxib, diclofenac and ibuprofen). BrdU assays were used to evaluate the effects of NSAIDs on the proliferation of OA chondrocytes. ELISA was used to detect the contents of pro-teoglycan and type-Ⅱcollagen in chondrocytes and culture supernatant. The differences of those indexes were compared be-tween groups. Results The positive rate of BrdU labelling index of chondrocytes increased significantly in ibuprofen group than that of other groups (P<0.05). The content of type-Ⅱcollagen in the culture supernatant increased significantly in di-clofenac group than that of other groups (P<0.05 or P<0.01). The content of proteoglycan in the culture supernatant in-creased significantly in ibuprofen group than that of other groups (P<0.05 or P<0.01). There were no significant differences in the content of type-Ⅱcollagen and proteoglycan between chondrocytes of NSAIDs groups (P>0.05). Conclusion Ibu-profen may stimulate the proliferation and the secretion of proteoglycan of human OA chondrocytes. Diclofenac may stimu-late the secretion of type-Ⅱcollagen of human OA chondrocytes. There were no effects of celecoxib on the proliferation and metabolism of human OA chondrocytes.
