Expression and Clinical Significance of miR-224 and miR-378e in Colorectal Cancer Tissues
10.3969/j.issn.0253-9896.2013.08.010
- VernacularTitle:miR-224和miR-378e在大肠癌组织中的表达及其临床意义*
- Author:
Lifei GAO
;
Yanfeng TIAN
;
Zengren ZHAO
;
Lijing ZHANG
;
Xinqi HE
;
Yongbin PEI
- Publication Type:Journal Article
- Keywords:
colorectal neoplasms;
carcinoma;
microRNAs;
oligonucleotide array sequence analysis;
polymerase chain reaction;
miR-224;
miR-378e
- From:
Tianjin Medical Journal
2013;(8):737-739
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and clinical significance of microRNA-224 and microRNA-378e in colorectal cancer tissues and normal mucosa adjacent to tumor lesions. Methods The gene chip technology was used to detect the different expression of miRNA in colorectal carcinoma tissues and adjacent normal tissues, which was then confirmed by real-time PCR. The relationship between the pathology and clinical data was analyzed. Results The expres-sion level of miR-224 was significantly up-regulated in tumor tissue, while miR-378e was down-regulated in tumor tissue, which was confirmed by real-time PCR. The expression of miR-224 was strongly associated with histological types, while miR-378e was strongly associated with the infiltration depth of colorectal cancer. Conclusion miR-224 is a potent tumor promoter, while miR-378e is a potent tumor suppressor. Both miR-224 and miR-378e can be used as potential colorectal cancer molecular markers.
