Effects of mechanical stretch preconditioning on endotoxin-induced damage to human type Ⅱ alveolar epithelial cells
10.3760/cma.j.issn.0254-1416.2015.03.023
- VernacularTitle:机械牵张预处理对内毒素诱发人肺泡Ⅱ型上皮细胞损伤的影响
- Author:
Ju GAO
;
Yang ZHANG
;
Xiaoping CHEN
;
Tao HUANG
- Publication Type:Journal Article
- Keywords:
Stress,mechanical;
Pulmonary alveoli;
Epithelial cells;
Endotoxemia
- From:
Chinese Journal of Anesthesiology
2015;35(3):352-354
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of mechanical stretch preconditioning on endotoxininduced damage to human type Ⅱ alveolar epithelial cells.Methods Human type Ⅱ alveolar epithelial cell line A549 ceils at the logarithmic growth phase were seeded in 96-well plates at a density of 0.5 × 106 cells/ml (2 ml/well).The cells were randomly divided into 3 groups:control group (group C,n =12);lipopolysaccharide (LPS) group (n =36) and 5％ CS + LPS group (n =48).The 5％ CS + LPS group was further divided into 4 subgroups,and A549 cells were exposed to 5％ cyclic stretch for 15,30,60 and 120 min in the 4 subgroups,respectively.LPS with the final concentration of 1 μg/ml was added after the end of mechanical stretch in the 4 subgroups,and A549 cells were incubated for 4 h.The apoptosis in cells was detected using flow cytometry and apoptosis rate was calculated.F-actin expression was determined using immunofluorescence.Results Compared with group C,the early and late apoptosis rates were significantly increased in LPS and 5％CS + LPS groups.Compared with the value at 4 h of LPS incubation in group LPS,the early and late apoptosis rates were significantly decreased,and the reconstruction of F-actin was reduced in 5％CS + LPS group.Conclusion Preconditioning with 5％ cyclic stretch (15-120 min) can mitigate endotoxin-induced damage to human type Ⅱ alveolar epithelial cells.
