Expression of matrix metalloproteinases-9 in the brain tissue of experimental hepatic encephalopathy rats
10.3760/cma.j.issn.1008-6706.2015.02.006
- VernacularTitle:肝性脑病大鼠脑组织中基质金属蛋白酶-9的表达变化及意义
- Author:
Lihua HU
;
Peng CHEN
;
Xiaoli WANG
;
Jiye ZHANG
;
Yanjie ZHANG
;
Peiyi ZHANG
- Publication Type:Journal Article
- Keywords:
Hepatic encephalopathy;
Blood-brain barrier;
Matrix metalloproteinases-9
- From:
Chinese Journal of Primary Medicine and Pharmacy
2015;22(2):175-177
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the significance of MMP-9 expression in the pathogenesis of hepatic encephalopathy.Methods 180 rats were randomly divided into the control group,hepatic encephalopathy 4,8 and 12h groups,45 rats in each group.In hepatic encephalopathy group,rats were intraperitoneally injected D-galactosamine (400mg/kg) with lipopolysaccharide (100μg/kg),and then the symptom was observed.Ammonia,the evans blue(EB) content,the brain water content and the MMP-9 mRNA in the brain tissue were assessed by real-time Q-PCR after 4,8 and 12h.Results 2h later the rats in hepatic encephalopathy groups began to appear the symptom.4 hours later,ammonia,the EB content,the brain water content and MMP-9 mRNA content of the hepatic encephalopathy groups began to increase [(74.27 ± 1.77) μ mol/L vs (33.85 ± 1.50) μ mol/L,F =5 983.36 ;(4.84 ±0.43)μg/g vs (3.96 ±0.48)μg/g,F =111.54; (78.63 ± 0.46)％ vs (76.07 ±0.39)％,F =737.25,and (1.037 ± 0.071) vs (0.829 ± 0.053),F =132.10,P ＜ 0.01],and with the extension of time increased obviously [(136.32 ± 3.88) μmol/L and (279.53 ± 9.78) μmol/L,F =5 983.36 ; (5.94 ± 0.35) μg/g and (6.71 ±0.50)μg/g,F=111.54;(80.50±0.32)％ and (82.14±0.30)％,F=737.25;(1.235 ±0.095) and (1.425± 0.113),F =132.10,P ＜ 0.01].Conclusion Blood brain barrier permeability enhancement and brain edema in hepatic encephalopathy rats may be related with MMP-9 mRNA increasing.
