The immunoregulatory effect of Rho kinase inhibitor Fasudil on macrophages in a mouse model of ;experimental autoimmune encephalomyelitis
10.3760/cma.j.issn.0254-5101.2015.05.003
- VernacularTitle:Fasudil 对实验性自身免疫性脑脊髓炎小鼠模型巨噬细胞的免疫调节作用研究
- Author:
Chunyun LIU
;
Shangde GUO
;
Jiezhong YU
;
Yanhua LI
;
Nianping ZHANG
;
Ling FENG
;
Zhi CHAI
;
Weijia JIANG
;
Baoguo XIAO
;
Cungen MA
- Publication Type:Journal Article
- Keywords:
Fasudil;
Experimental autoimmune encephalomyelitis;
Macrophage;
Anti-inflamma-tion
- From:
Chinese Journal of Microbiology and Immunology
2015;(5):335-340
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the immunoregulatory effect of Fasudil-modified macrophages on cell transferred experimental autoimmune encephalomyelitis ( EAE) in a mouse model.Methods Fe-male C57BL/6 mice were immunized with MOG35-55 to establish the model of EAE.The encephalomyelitic mononuclear cells ( MNCs) were isolated from spleen of mice with EAE on day 9 after immunization and treated with or without Fasudil for 72 h in vitro.Several assays including the flow cytometry analysis, Griess reaction and ELISA were performed to analyze the M1 and M2 phenotypes of macrophages, the production of NO and the levels of cytokines, respectively.The cultured MNCs (5×107 cells) were resuspended in 500μl of PBS and transferred into na?ve C57BL/6 recipients via intraperitoneal injection.Two groups including the PBS-MNCs group and the Fasudil-MNCs group were set up.The body weights and clinical scores of the mice in each group were recorded in every other days after the induction of EAE in the recipients.Results The Fasudil treated MNCs affected the induction of EAE in adoptive cell transferred mice.The expression of CD16/32, iNOS and IL-12 on F4/80-macrophages were decreased, while the expression of CD206, CD23 and IL-10 on F4/80-macrophages were increased upon the treatment of Fasudil, indicating that Fasudil im-proved the differentiation of macrophages from M1 to M2 phenotypes.Moreover, Fasudil inhibited the pro-duction of NO and enhanced the expression of Arginase-1.Conclusion Fasudil ameliorated the clinical se-verity of EAE in mice by promoting the transformation of macrophages from M1 to M2 phenotype.
