Function Study of the Nano-Molecular PAMAM as miR Carrier Targeting Gastric Adenocarcinoma
10.3969/j.issn.0253-9896.2013.12.003
- VernacularTitle:纳米分子PAMAM作为miR药物载体靶向胃腺癌的功能研究
- Author:
Wei SONG
;
Zhongmin JIANG
;
Ping XU
;
Yinghong LIANG
;
Xiaozhi LIU
- Publication Type:Journal Article
- Keywords:
gastric adenocarcinoma;
liposomes;
microRNA;
nanomaterials;
polyamide-amine
- From:
Tianjin Medical Journal
2013;(12):1147-1149
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the function of the nano-molecular polyamide-amine (PAMAM) as microRNA(miR) carrier targeting gastric adenocarcinoma, and the foundation of developing an efficient delivery of small molecule drugs tar-geting gastric cancer thereof. Methods The folic acid (FA)/PAMAM comoles compound was prepared by dialysis method. After transfection of miR-7 or liposomes into SGC-7901 cell line, fluorescence microscope was used to detect the gene trans-fect efficiency. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the miR-7 level. The immu-nocytochemistry assay was used to test the protein expressions of epidermal growth factor receptor (EGFR), protein kinase B (PKB) and proliferating cell nuclearantigen (PCNA). The transwell system was utilized to explore the migration ability of tu-mor cells. Results Compared with liposme, FA/PAMAN complex compound can significantly improve the level of miR-7 in SGC-7901 cells,reduce the protein levels of EGFR, PKB and PCNA in SGC-7901 cells, and also reduce the percentage of cancer cell migration (P<0.05). Conclusion PAMAM can effectively transfect miR into gastric cancer cells, which is expected to become an efficient delivery of small molecule drugs.
