18F-FDG effects on maturation and immune function of dendritic cells in vitro
10.3969/j.issn.2095-4344.2014.42.007
- VernacularTitle:树突状细胞体外成熟及免疫功能的18F-FDG评价
- Author:
Xiao LEI
;
Yingkui LIANG
;
Wenrui ZHAO
;
Sheng WANG
;
Feng GUO
;
Qiang FU
- Publication Type:Journal Article
- Keywords:
dendritic cells;
chemotactic factors;
immunity
- From:
Chinese Journal of Tissue Engineering Research
2014;(42):6758-6762
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Previous studies have shown that different doses of ionizing radiation may have some impact on maturation and immune function of dendritic cells. OBJECTIVE:To evaluate the effect of 18 F-FDG with different concentrations on maturation and immune function of dendritic cells from human peripheral blood mononuclear cells in vitro. METHODS:Human peripheral blood mononuclear cells-derived mature dendritic cells were divided into five groups:PBS, 92.5×10 4, 185×104, 370×104, 740×104 Bq/mL 18 F-FDG were separately added into each group. Dendritic cells were col ected 24 hours later. Apoptosis rate, phenotypic expression (CD1α, CD80, CD83, CD86, HLA-DR), ability of mixed lymphocyte reaction and expression of macrophage inflammatory protein 1αand monocyte chemotactic factor-1 in cellculture supernatant were detected. RESULTS AND CONCLUSION:Apoptosis rate, phenotypic expression of CD86, ability of mixed lymphocyte reaction and expression of monocyte chemotactic factor-1 were down-regulated after culture in 740×10 4 Bq/mL 18F-FDG. However, 18F-FDG at other concentrations had no influence on maturation and immune function of dendritic cells. This study demonstrates that low-concentration 18F-FDG has little influence on apoptosis, maturation, antigen presentation, and migratory capacity of dendritic cells, and it may be selected at an appropriate concentration as a label for dendritic cells in vitro.
