Three kinds of engineered hepatic tissues constructed using rat bone marrow mesenchymal stem cells
10.3969/j.issn.2095-4344.2014.41.004
- VernacularTitle:三种大鼠骨髓间充质干细胞源性人工肝组织的构建与评价
- Author:
Jianxiong YU
;
Jing YUAN
;
Ruiyun XU
- Publication Type:Journal Article
- Keywords:
bone marrow;
mesenchymal stem cells;
liver failure,acute;
liver,artificial
- From:
Chinese Journal of Tissue Engineering Research
2014;(41):6578-6584
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Engineered hepatic tissue is considered a promising strategy for healing acute liver failure. But, there are series of hindrances in the construction of engineered hepatic tissues, including acquisition of vital hepatocytes, choice of scaffolds and culture system, and nutrition supply. OBJECTIVE:To construct three kinds of engineered hepatic tissues in hope to screen the optimal one for transplantation in acute liver failure. METHODS:After purification, amplification, bone marrow mesenchymal stem cells were induced to differentiate into hepatocyte-like cells which were co-cultured with acellular amniotic membrane, DACRON PATCH cardiovascular surgical patch, biological surgical patch, respectively to construct three kinds of engineered hepatic tissues. After 3 days of culture, morphological and functional detections were performed. RESULTS AND CONCLUSION:Rat bone marrow mesenchymal stem cells with higher purity were successful y harvested by using density gradient centrifugation and adherent methods, and then the cells were differentiated into hepatocyte-like cells. In the three kinds of engineered hepatic tissues, hepatocyte-like cells were found to be combined with the biological surgical patch to the maximum extent, and their combination exhibited stronger ability of urea synthesis and albumin secretion, which provides experimental basis for treatment of acute liver failure.
