Roles of BCG in establishing collagen-induced arthritis mice
10.3969/j.issn.1000-484X.2014.10.007
- VernacularTitle:卡介苗在关节炎模型小鼠诱导中的作用
- Author:
Ruimin LIU
;
Bo LIN
;
Shibai ZHU
;
Huiling BAI
;
Yuanfang MA
- Publication Type:Journal Article
- Keywords:
CIA mice;
BCG;
Freund′s complete adjuvant;
CIA-related features
- From:
Chinese Journal of Immunology
2014;(10):1330-1333
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the correlation of BCG concentration in Freund complete adjuvant ( FCA) and severity of arthritis during arthritis establishment in DBA 1/j male mice induced by bovine type Ⅱcollagen.Methods:CIA was induced by the im-munization of DBA1/j mice with bovine type Ⅱcollagen and BCG of various concentrations dissolved in FCA.To ascertain the effects administering the collagen booster,CIA-related features(including body weight,clinical scoring of arthritis),TNF-αin serum and the histopathological changes in the spleen and the joints regions were measured.Results:4 mg/ml BCG induced more serious arthritis than 1 mg/ml in DBA1/j mice after collagen exposure.Conclusion:There is a positive correlation of arthritis severity with BCG.Which indicates that selection of adjuvant with suitable BCG concentration could determine pathological outcome in CIA mouse model .
