Roles of UⅡ/UT system played in innate immune inflammatory signal pathway TLR4-IRF3 in LPS-stimulated primary Kupffer cells
10.3969/j.issn.1000-484X.2014.10.004
- VernacularTitle:UⅡ／UT系统对LPS刺激枯否细胞固有免疫炎症信号通路TLR4-IRF3的影响
- Author:
Wenjuan TU
;
Xiaoting WANG
;
Liangming LIU
;
Tong ZHU
;
Dongyu LIANG
;
Zhiwen YANG
;
Deyong GAO
- Publication Type:Journal Article
- Keywords:
Kupffer cell;
Inflammation;
Lipopolysaccharide;
Urotensin Ⅱ;
Urantide;
TLR4;
IRF3
- From:
Chinese Journal of Immunology
2014;(10):1313-1319
- CountryChina
- Language:Chinese
-
Abstract:
Objecitve:To investigate effects of urotensin Ⅱ( UⅡ)/UT system on innate immune inflammatory signal pathway TLR4-IRF3 in the lipopolysaccharide (LPS)-stimulated Kupffer cells (KCs).Methods: Rat KCs were isolated and cultured.Pro-in-flammatory cytokines including IL-6,IFN-βand IFN-γwere assayed by ELISA in culture supernatant of KCs.Cell surface TLR4 were tested with flow cytometry technique.Expression of IRF3 were tested with real-time PCR and Western blot.Results: Significant increases were showed in IL-6, IFN-βand IFN-γsecretion, TLR4-expressed positive rates and IRF3 mRNA levels in KCs after stimulated by LPS,but were inhibited via urantide pretreatment.In addition,LPS induced upregulation of nuclear IRF3 protein and downregulation of cytoplasm IRF3 protein in KCs,which were blocked by urantide pretreatment.Conclusion:UⅡ/UT system mediates immune inflammatory response in part through activating TLR 4-IRF3 pathway in LPS-stimulated KCs.
