miR-451 inhibits migration and invasion of gastric cancer cells by targeting migration inhibitory factor
10.3760/cma.j.issn.1008-1372.2014.09.006
- VernacularTitle:miR-451通过靶向调控MIF抑制胃癌细胞的迁移与侵袭
- Author:
Xuemei ZHANG
;
Yanliang SUN
;
Zhiyong YAO
;
Ximei WANG
- Publication Type:Journal Article
- Keywords:
MicroRNAs;
Stomach neoplasms/pathology;
Stomach neoplasms/genetics;
Macrophage migration-inhibitory factors;
Cell movement;
Neoplasm invasiveness
- From:
Journal of Chinese Physician
2014;16(9):1172-1175
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore whether miR-451 suppresses cell migration and invasion by targeting macrophage migration inhibitory factors (MIF),thus to reveal molecular mechanism that miR-451 functions as a tumor suppressor in gastric cancer.Methods A quantitative real-time polymerase chain reaction (qRT-PCR) was carried out to detect the expression of miR-451 in gastric cancer and normal stomach mucosa.Western blotting was performed to detect the expressions of MIF protein regulated by miR-451 in MGC 803 cells.The migration and invasion abilities of MGC-803 cell were evaluated with wound-healing and transwell invasion assays.Results miR-451 was down-regulated in gastric cancer tissues.The expressions of miR-451 were negatively correlated with the clinical stage and lymph node metastasis in gastric cancer patients.Western blot showed that a notable reduction of the protein level of MIF by restoring miR-451 in MGC-803 cells.Overexpression of miR-451 inhibited the migration and invasion of MGC-803 cells.Conclusions miR-451 suppresses cell migration and invasion by targeting MIF in gastric cancer.
