Expression of TSSC1 in Glioma Tissue and Its Effect on Cell Biological Behavier of Glioma U87 Cells

VernacularTitle:胶质瘤组织中TSSC1的表达及其对胶质瘤U87细胞生物学行为的影响
Author: Yibin LIU ; Yunfu MA ; Jianshe CHEN ; Yinhui WANG
Publication Type:Journal Article
Keywords: glioma; RNA,small interfering; TSSC1; U87 cell
From: Tianjin Medical Journal 2014;(6):540-543
CountryChina
Language:Chinese
Abstract: Objective To evaluate the diagnostic value of TSSC1 in glioma patients and its influence on cell biologi-cal behavior of glioma U87 cells. Methods RT-PCR and Western blot were used to examine the expression of TSSC1 in glioma samples, including 80 normal paraneoplastic tissues and 80 primary tumors. MTT and transwell were used to analyze the effect of TSSC1 knockout on proliferation, migration, and invasion in U87 cells. Results TSSC1 is down-regulated in glioma compared to its paraneoplastic counterparts and negatively related to higher grade. Furthermore, knockdown of TSSC1 expression results in increased proliferation, migration and invasion in U87 cells in vitro. Conclusion Our results may worked as a marker for early diagnosis and prognosis of glioma.