Effect of Doxycycline on Gastric Cancer Cell Proliferation and Notch1, MMP-9 Expression
10.3969/j.issn.0253-9896.2014.06.006
- VernacularTitle:强力霉素对胃癌细胞增殖及Notch1、MMP-9表达的影响
- Author:
Min XU
;
Hong LI
- Publication Type:Journal Article
- Keywords:
stomach neoplasms;
cell line,tumor;
receptor,Notch1;
matrix metalloproteinase 9;
cell proliferation;
cell cycle;
DOXYCYCLINE
- From:
Tianjin Medical Journal
2014;(6):533-535
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Doxycycline on gastric cancer cell line BGC-823 proliferation and Notch1, Matrix metalloproteinase-9(MMP-9)expression in order to shine a light on new anticancer drugs. Methods Final concentration of 0, 5, 10, 20, 40 mg/L doxycycline were added into human gastric cancer cell line BGC-823. Cell pro-liferation was detected by MTT;Cell cycle distribution was assessed by flow cytometry;Notch1, MMP-9 protein expression was revealed by Immunoblot. Results Doxycycline can inhibit proliferation of human gastric cancer cells line BGC-823, and its effect is dose and time-dependent(P＜0.01). Doxycycline alters distribution of gastric cancer cell line BGC-823. With increasing drug concentration, the proportion of cells in S phase dropped(P＜0.01). Notch1 expression rose and MMP-9 expression decreased(P＜0.01). Conclusion Doxycyclinecan inhibited gastric cancer cell line BGC-823 prolif-eration and up-regulating Notch1 might be one mechanisms.
