Effect of Intracerebroventricular Leptin Injection on Limbs Fracture Healing
10.3969/j.issn.0253-9896.2014.06.013
- VernacularTitle:脑室内注射瘦素对四肢骨折愈合的影响
- Author:
Baolong LIU
;
Zhiming SUN
;
Qiaoli WU
;
Hong WANG
;
Hua YAN
- Publication Type:Journal Article
- Keywords:
leptin;
cerebrospinal fluid;
tibial fracture;
fracture healing;
bone density;
in siut hybridization,fluores-cence;
rabbits;
bone morphogenetic protein 2
- From:
Tianjin Medical Journal
2014;(6):558-560
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the enhanced osteogenesis during bone fracture healing after intracerebroven-tricular (ICV) leptin injection, using rabbit model with created segmental bone defect in right tibial. Method Segmental critical-sized defects were created at the right tibial bone of skeletally mature New Zealand white rabbits. In experiment group (leptin group), recombinant rabbit leptin was injected into cerebellomedullary cistern through foramen magnum. While in control group, normal saline was injected in the same way. Bone Mineral Density (BMD) was evaluated by qCT at 1st, 3rd, 7th, 14th and the 21st days. At the 21st days, all rabbits were euthanized to collect the right tibia for histomorphology, to ex-amine the BMP-2 expression in the bone callus by Fluorescence in situ hybridization (FISH). Result In the leptin group, body weight declined more obviously than control group then it start to arise at the 14th day;qCT showed significant higher BMD in leptin group than in control group at the 7th day;FISH showed a higher BMP-2 expression in leptin group than in control group. Conclusion Cerebellomedullary leptin injection through foramen magnum could accelerate limb fracture healing in rabbit model with right tibial bone defection.
