The expression of MMP-1 and MMP-2 of periodontal ligament fibroblasts treated by tea polyphenols and LPS
10.3969/j.issn.1001-3733.2014.06.08
- VernacularTitle:茶多酚对脂多糖介导下人牙周膜成纤维细胞 MMP-1、MMP-2表达的影响
- Author:
Xiaona LI
;
Qin FAN
;
Weikun HUANG
;
Xiaoyan GUAN
;
Di ZHANG
;
Ningjing KONG
;
Guohui BAI
;
Jianguo LIU
- Publication Type:Journal Article
- Keywords:
Tea polyphenols;
Lip polysaccharide;
Human periodontal ligament cells(HPDLCs);
Matrix metal-loproteinases-1(MMP-1);
Matrix metalloproteinases-2(MMP-2)
- From:
Journal of Practical Stomatology
2014;(6):774-777
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To survey the expression of MMP-1,MMP-2 of human periodontal ligament cells(HPDLCs)treated by tea polyphenols(TP)and lipopolysaccharide(LPS).Methods:HPDLCs were in vitro cultured in vitro and treated by TP(200 μg/ml) and /or LPS(100 μg/ml)for 24,48 and 72 h respectively,the secretion of MMP-1 and MMP-2 were examined by ELISA,MMP-1 and MMP-2 mRNA expression was examined by real-time PCR.Results:The secression and mRNA expression of MMP-1 and MMP-2 of HPDLCs increased by LPS treatment and significantly inhibited by TP at the different times.Conclusion:TP can inhibit the col-lagen degradation of HPDLCs mediated by LPS.