Modified platelet-rich plasma promotes the osteogenic differentiation of stem cells from human exfoliated deciduous teeth

VernacularTitle:改良富血小板血浆促进乳牙牙髓干细胞成骨分化作用研究
Author: Jun WEN ; Jianmin DUAN ; Hongtao LI ; Sihan LI ; Xin LI ; Buling WU
Publication Type:Journal Article
Keywords: Plateletrich plasma; Stem cells from human exfoliated deciduous teeth; Mesenchymal stem cells; Osteogenic differentiation
From: Journal of Practical Stomatology 2014;(6):805-808
CountryChina
Language:Chinese
Abstract: Objective:To investigate the effect of modified plateletrich plasma(mPRP)on the osteogenic differentiation of stemcells from human exfoliated deciduous teeth(SHED).Methods:mPRP at 1%,2%,5%,10% and FBS at 10% were added to thecultured SHED of passage 4,respectively.The influence of mPRP on alkaline phosphatase(ALP)activity was evaluated using ALPkit.RUNX2 and osteocalcin mRNA expression in the treated cells were examined by realtime PCR.Results:mPRP enhanced ALPactivity in the SHED,and the effect of mPRP was more obvious at 2%.Treatment of the cells with 2% mPRP upregulated the mRNAexpressions of RUNX2 and osteocalcin.Conclusion:mPRP can promote the osteogenic differentiation of SHED.