Establishment and identification of a ClC-3/MMTV-PyMT hybrid mouse strain
10.3969/j.issn.1005-4847.2014.06.003
- VernacularTitle:CLCN3／MMTV－PyMT 转基因小鼠杂交群体的建立及鉴定
- Author:
Lulu DENG
;
Qin LI
;
Hui WU
;
Jianwen MAO
;
Bin XU
- Publication Type:Journal Article
- Keywords:
CLCN3;
MMTV-PyMT;
Transgenic mice;
Breast cancer
- From:
Acta Laboratorium Animalis Scientia Sinica
2014;(6):17-21
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a CLCN3/MMTV-PyMT double transgenic mouse model of spontaneous breast tumor with simultaneously overexpressing ClC-3.Method CLCN3 transgenic mice were crossed with MMTV-PyMT spon-taneous mammary tumor model mice.The genotype was determined by PCR.The expression of ClC-3 in tissues was detec-ted by immunofluorescence and Western blot.Results CLCN3 and MMTV-PyMT transgenic mice were bred and CLCN3/MMTV-PyMT hybrid mouse model was successfully established.The ClC-3 expression in CLCN3/MMTV-PyMT hybrid mice was higher than that in the MMTV-PyMT mice, assessed by immunofluorescence and Western blot analysis.Conclu-sions Transgenic mouse models of spontaneous breast cancer with simultaneously overexpressing ClC-3 are successfully es-tablished.The double transgenic mice provide a good animal model for further research of ClC-3 in tumor growth and metas-tasis.
