The influence of HGF on the expression of VEGF-C and its mechanism in human tongue squamous cell carcinoma Tca8113 cells
10.3969/j.issn.1001-3733.2014.06.012
- VernacularTitle:肝细胞生长因子对人舌鳞癌 Tca8113细胞 VEGF-C 表达的影响及作用机制的初步研究
- Author:
Hongliang DU
;
Dengqi HE
;
Zhirui ZHANG
;
Jilian XU
;
Yaming XI
- Publication Type:Journal Article
- Keywords:
Oral squamous cell carcinoma;
HGF;
VEGF-C;
PI3K/Akt;
P44/P22MAPK
- From:
Journal of Practical Stomatology
2014;(6):792-795
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence of hepatocyte growth factor(HGF)on the expression of vascular endothelial growth factor C(VEGF-C)and the mechanism of HGF-induced VEGF-C expression in tongue squamous cell carcinoma Tca8113 cells.Methods:Tca8113 cells were cultured and exposed to HGF with various concentrations.The expression level of VEGF-C was assessed by ELISA.Signaling transduction inhibitors LY294002,U0126,SP600125,SB203580 was used to block PI3K/Akt,P44 /P22MAPK,JNK,P38MAPK signaling pathways,respectively.Then,the expression level of VEGF-C was detected by ELISA.Re-sults:The VEGF-C expression of Tca8113 cells increased at the beginning and decreased later with the increase of HGF concentra-tion.When the concentration of HGF was 40 ng/ml,VEGF-C expression level was the highest.Inhibitor LY294002 of PI3K/Akt and Inhibitor U0126 of P44 /P22MAPK significantly blocked the effects on HGF-induced VEGF-C up-regulation(P ＜0.01 ).Inhibitor SP600125 of JNK and inhibitor SB203580 of P38MAPK didn't interfere HGF-induced VEGF-C expression(P >0.05).Conclusion:HGF contributed to the expression of VEGF-C,PI3K/Akt and P44 /P22MAPK signaling pathways may be involved in HGF-induced VEGF-C up-regulation,and may play potential roles in lymphatic metastasis of oral squamous cell carcinoma.
