Effect of “Double Reinforcing and One Unblocking” Electroacupuncture on Hepatic Mitochondrial Structure and ATPase Activity in a Rat Model of Senile Yang Deficiency
10.13460/j.issn.1005-0957.2015.01.0063
- VernacularTitle:“双固一通”电针法对老年阳虚证模型大鼠肝线粒体结构及ATP酶活性的影响
- Author:
Jianmin LIU
;
Jingfang LV
;
Hua WANG
;
Jing LIU
;
Mingyang CHEN
;
Jingzhi WANG
- Publication Type:Journal Article
- Keywords:
Acupuncture therapy;
Electroacupuncture;
Yang deficiency syndrome;
Hepatic mitochondria;
ATPase;
“Double reinforcing and one unblocking”electroacupuncture;
Rats
- From:
Shanghai Journal of Acupuncture and Moxibustion
2015;(1):63-66
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of “double reinforcing and one unblocking” electroacupuncture on hepatic mitochondrial structure and ATPase activity in a rat model of senile yang deficiency. Methods Forty 5-month-old male SD rats were selected and randomly allocated to groups A, B, C and D, 10 rats each. Group A was a normal control group. A model of senile yang deficiency was made by 40 days’ subdermal injection of D-galactose and then 7 days’ intramuscular injection of hydrocortisone in the other three groups of rats. Group C received treatment with “double reinforcing and one unblocking”electroacupuncture at points Guangyuan(CV4), Zusanli(ST36) and Baihui(GV20); group D, treatment with electroacupuncture at points Zhongji(CV3), Yinlingquan(SP9) and Yintang(GV29). Treatment was given 6 times a week for a total of 4 weeks. After treatment, the anti-fatigue ability was determined by swimming exhaustion time, hepatic mitochondrial structure was observed under a transmission electron microscope and hepatic Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities were measured by colorimetry in every group of rats. Results The mean swimming exhaustion time shortened significantly (all P＜0.01); hepatic mitochondrial inner and outer membranes were blurred, mitochondrial ridges deformed, ruptured or disappeared and mitochondria swelled; both Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities decreased (P＜0.01, P＜0.05) in groups B, C and D of rats compared with group A. Swimming exhaustion time lengthened significantly (P＜0.01), hepatic mitochondrial structure improved significantly and both Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities increased significantly (P＜0.01, P＜0.05) in group C compared with group B. Rat mean swimming exhaustion time shortened (P＜0.05), hepatic rough endoplasmic reticula degranulated and hepatic Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities decreased (P＜0.05) in group D compared with group B. Conclusion “Double reinforcing and one unblocking”electroacupuncture can improve anti-fatigue ability, ameliorate hepatic mitochondrial ultrastructure, increase hepatic Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities and promote hepatic energy metabolism in a rat model of senile yang deficiency.
