Effects of Heat-Sensitive Moxibustion on Antioxidative Capacity in Rat Model of Focal Cerebral Ischemia-Reperfusion Injury
10.3969/j.issn.0253-9896.2014.01.016
- VernacularTitle:热敏灸对脑缺血再灌注损伤大鼠SOD、MDA的影响
- Author:
Aijiao XIAO
;
Rixin CHEN
;
Mingfei KANG
;
Hao ZHANG
- Publication Type:Journal Article
- Keywords:
brain ischemia;
reperfusion injury;
superoxide dismutase;
malondialdehyde;
acupoint sticking therapy;
rats,Sprague-Dawley;
heat-sensitive moxibustion
- From:
Tianjin Medical Journal
2014;(1):51-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of heat-sensitive moxibustion on the expression and activity of super-oxide dismutase (SOD) and the content of malondialdehyde (MDA) in focal cerebral ischemia-reperfusion injury in rats, and the mechanism thereof. Methods Thirty-six male SD rats were randomly divided into four groups, sham-operated group (n=6), ischemia-reperfusion (I/R) injury group (n=10), I/R injury with 15-minute moxibustion group (n=10) and I/R injury with 35-minute moxibustion group (n=10). The focal cerebral ischemia-reperfusion rat model was induced by middle cere-bral artery occlusion (MCAO) for 2 h followed by reperfusion. Values of SOD activity and MDA content were determined by colorimetry, and the cortical expression of SOD2 protein was detected by Western blot technique. Results Values of SOD activities were significantly higher in serum (22.78±1.31)U/mL and cortex (4 909.6±1 345.6) U/g of heat-sensitive moxibus-tion group than those of model group (20.17±1.12)U/mL and (2 602.0±1 515.5)U/g. Values of MDA contents were significant-ly decreased in serum (3.78±2.00)μmol/L and cortex (1 226.5±38.4)nmol/g in heat-sensitive moxibustion group than those of model group (16.82 ± 6.70)μmol/L and (1 905.6 ± 478.6) nmol/g. The cortical expression of SOD2 protein (0.974 ± 0.166) was higher in heat-sensitive moxibustion group than that of model group (0.702±0.040). Conclusion Heat-sensitive moxi-bustion could reduce the damage of cerebral inchemia-reperfusion, which might be through improving SOD activity, increas-ing SOD expression and decreasing MDA content.
