Establishment and Application of Hyperinsulinemic-Euglycemic Clamp Technique in Conscious Rats
10.3969/j.issn.0253-9896.2014.01.019
- VernacularTitle:清醒大鼠高胰岛素-正血糖钳夹技术的建立和应用
- Author:
Yuehua FENG
;
Guanghua LUO
;
Lixin CHEN
- Publication Type:Journal Article
- Keywords:
hyperinsulinism;
fatty acids,nonesterified;
insulin resistance;
hyperinsulinemic-euglycemic clamp tech-nique;
glucose infution rate;
nonesterified fatty acid
- From:
Tianjin Medical Journal
2014;(1):62-64
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the hyperinsulinemic-euglycemic clamp(HEC) technique in conscious rats, and to explore the effect of acute infusion of lipid on glucose infusion rate (GIR) in rats. Methods Ten SD rats were random-ly divided into two groups, 5 rats for each group. The right jugular vein and left carotid artery were catheterized and under-went a HEC with infusion of lipid (intralipid group) for 6 hours, and with continuing infusion of 5%glucose (control group). The plasma levels of free fatty acid(FFA) and GIR were measured by HEC method. Results The level of FFA concentration increased by 17.6-fold, and GIR was reduced by 27%in the intralipid group compared to those of control group (P＜0.001). Conclusion The rat model of HEC has been successfully established by intravenous intralipid infusion, which can be con-firmed by HEC technique.
