The Effect of Pseudolaric Acid on the Expression of VEGF in Hepatocellular Carcinoma Cells
10.3969/j.issn.0253-9896.2014.01.004
- VernacularTitle:土槿乙酸对人肝癌细胞中血管内皮生长因子表达的影响
- Author:
Ping ZHU
;
Lianyi GUO
- Publication Type:Journal Article
- Keywords:
cell line,tumor;
liver neoplasms;
vascular endothelial growth factors;
reverse transcriptase polymerase chain reaction;
blotting,western;
pseudolaric acid B
- From:
Tianjin Medical Journal
2014;(1):10-12
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of pseudolaric acid B(PAB) on the expression of vascular endothelial growth factor (VEGF) in human hepatoma BEL-7402 cells. Methods BEL-7402 cells were cultured with PAB (0, 0.5, 1.0, 2.0, 4.0 and 8.0μmol · L-1) for 6 h and stained with 0.4%trypan blue. The cell viability was observed and calculated under the microscope. The cell proliferation was detected by WST-8 analysis. The expression of VEGF mRNA in BEL-7402 cells was detected using RT-PCR method. The expression of VEGF protein in BEL-7402 cells was detected using Western blot assay. Results There was no significant difference in the cell proliferation of BEL-7402 cells between different PAB treat-ment groups and control group. With the increased concentration of PAB, the expressions of VEGF mRNA were significantly decreased in different PAB treatment groups (1.90±0.02,1.31±0.03,0.86±0.04,0.60±0.03 and 0.34±0.02) compared with that of control group (2.53±0.07, P＜0.01). The expressions of VEGF protein were significantly decreased in different PAB treat-ment groups (2.60±0.09, 2.42±0.07, 1.85±0.05, 0.92±0.05 and 0.60±0.04) compared with that of control group (3.06±0.15, P＜0.01). Conclusion PAB can inhibit the expressions of VEGF mRNA and protein in BEL-7402 cells.
