Molecular detection of spotted fever group rickettsia in Dermacentor silvarum from the Xunke Area,China
10.3969/cjz.j.issn.1002-2694.2014.10.007
- VernacularTitle:黑龙江逊克地区森林革蜱斑点热立克次体 DNA的检测
- Author:
Li FENG
;
Zuo WANG
;
Jun YANG
;
Miao YU
;
Liqiang WANG
;
Guangbin QIU
;
Rubo ZAI
;
Yimin WU
- Publication Type:Journal Article
- Keywords:
ticks;
spotted fever group rickettsia;
PCR;
sequence analysis
- From:
Chinese Journal of Zoonoses
2014;(10):1020-1023
- CountryChina
- Language:Chinese
-
Abstract:
To identify the prevalence and the distribution of ticks infected with spotted fever group rickettsiae (SFGR) in Xunke Area of Heilongjiang Province ,China ,partial outer membrane protein A gene (ompA) and citrate synthase gene (gltA) specific fragments were assessed using the PCR method .The positive products were sequenced .Result showed that the pres-ence of SFGR was 14 of 60 in detection Dermacentor silvarum cases ,while the overall positive rate was 23 .33% .Its nucleotide sequence of ompA showed 99 .3% and 99 .18% similarity with nucleotide sequence of Rickettsia sp .JL-02 and Rickettsia rao-ultii respectively .And the evolutionary positions of SFGR species were similar with Rickettsiamontana and Rickettsiamassili-ae .It's concluded that the nature focus of tick-borne spotted fever did exist in the area of Xunke Area of Heilongjiang Province , China .
