Inhibitory Effects of 1, 25-Dihydroxy-Vitamin D3 on Differentiation of Adipocytes
10.3969/j.issn.0253-9896.2013.10.011
- VernacularTitle:1,25-二羟基维生素D3抑制脂肪细胞分化作用的研究
- Author:
Xiaohui GUAN
;
Jun WANG
;
Fei GUO
;
Jie ZHOU
;
Baoli WANG
- Publication Type:Journal Article
- Keywords:
adipocytes;
cell differentiation;
calcitriol;
PPAR gamma;
CCAAT-enhancer-binding protein-alpha;
beta catenin;
adipocyte characterization factor aP2
- From:
Tianjin Medical Journal
2013;(10):981-984
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of 1, 25-dihydroxy-vitamin D3 (1, 25 (OH)2D3) on adipocyte differen-tiation and the underlying mechanism. Methods The mesenchymal stem cell line C3H10T1/2 was randomly divided into 6 groups including control group, differentiation group and 4 different doses of 1, 25(OH)2D3 groups. The control group was treated with vehicle. The differentiation group was supplemented with adipocyte differentiation reagent. And the 1,25(OH)2D3 groups were treated with adipocyte differentiation reagents and 10-9, 10-8, 10-7 and 10-6 mol/L of 25(OH)2D3. After culturing for 5 days, the cells were stained with oil red O, and the expression levels of adipocyte-specific transcription factors and Wnt/β-catenin signaling pathway related genes were examined by RT-PCR or Western blot methods. Results 1,25(OH)2D3 sig-nificantly reduced the number of differentiated adipocytes and blocked the mRNA levels of adipocyte specific transcription factor PPARγ(peroxisome proliferator-activated receptor gamma), C/EBPα(CCAAT enhancer binding proteinα) and adipo-cyte characterization factor aP2 (fatty acid binding protein 4). These were paralleled by the decreased mRNA expression of Wnt/β-catenin signaling pathway inhibitor sFRP1 (Secreted frizzled-related protein 1) and the increased level ofβ-catenin protein. Conclusion 1, 25(OH)2D3 inhibits adipocyte differentiation, which may be related to the activation of Wnt/β-catenin signaling.
