Investigation of point mutations in pbp1a gene of streptococcus pneumoniae resistant strains by PCR-SSCP-sequencing
- Author:
Phan Quoc Viet
- Publication Type:Journal Article
- Keywords:
Mutation
- MeSH:
Streptococcus pneumoniae;
Mutation;
Genes
- From:Journal Ho Chi Minh Medical
2004;8(2):103-107
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Study used the methods of PCR-SSCP-sequencing to detect and identify point mutations in pbp1a gene, one of the genes involved in penicillin resistance of streptococcus pneuminiae. SSCP analysis result of PCR products from 19 clinical resistant and 1 susceptible strains allowed their classification into defferent groups depending on their electrophoretic pattern. This classification fits results obtained by sequencing. With improvement in the resolution capacity of SSCP, these methods could be used efficiently to investigate penicillin resistance in streptococcus pneuminiae
