Correlation of trace element zinc with bone morphogenetic protein 7 and Stro-1+cells in proximal femur:predicting hip prosthesis life
10.3969/j.issn.2095-4344.2014.44.002
- VernacularTitle:股骨近端微量元素Zn与骨形态发生蛋白7及Stro-1+细胞的相关性：预测髋关节假体寿命
- Author:
Xiaodong FU
;
Weili WANG
;
Yi SHEN
;
Xiaomiao LI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2014;(44):7061-7065
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:The correlation of zinc with Stro-1+cells and bone morphogenetic protein 7 surrounding the prosthesis may affect the bone fusion and survival rate in hip prosthesis. ＜br> OBJECTIVE:To analyze the correlation of zinc content with Stro-1+cells and bone morphogenetic protein 7 in proximal femur. ＜br> METHODS:Bone samples were obtained from the discarded metaphysis region of the proximal femur in 24 patients with primary total hip replacement. Bone marrow mesenchymal stem cells were cultured in vitro. At 14 days after culture, Stro-1+cells in bone marrow mesenchymal stem cells were detected using flow cytometry. Bone morphogenetic protein 7 expression in cellsupernatant was detected using enzyme-linked immunosorbent assay. Zinc content in supernatant was measured using radio-immunity assay. ＜br> RESULTS AND CONCLUSION:No significant difference in the zinc content was detected in different age groups and different gender groups. Zinc content was positively associated with Stro-1+cells and bone morphogenetic protein 7 expression. The further study of the trace element zinc in proximal femur can predict and intervene the longevity of hip prosthesis.
