The Impact of Peng-Hui on the Expression of IRS-1 in Pancreas and Skeletal Muscle of Type 2 Diabetic Rats
10.3969/j.issn.0253-9896.2013.09.009
- VernacularTitle:蓬灰对2型糖尿病大鼠胰腺及骨骼肌IRS-1表达的影响*
- Author:
Rui ZHAO
;
Dan QIANG
;
Xiaomin XIE
- Publication Type:Journal Article
- Keywords:
diabetes mellitus,type 2;
pancreas;
muscle,skeletal;
blood glucose;
insulin;
rats,Sprague-Dawley;
insu-lin receptor substance 1;
Peng-Hui
- From:
Tianjin Medical Journal
2013;(9):878-880
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of different doses of Peng-Hui on expression levels of insulin receptor substance 1 (IRS-1) in type 2 diabetes (T2DM) rats. Methods T2DM rat model was established by feeding with high-fat-diet and intraperitoneal injecting streptozotocin (STZ). T2DM model rats were randomized into four groups: T2DM control group, high dose Peng-Hui (150 mg/kg) group, medium dose Peng-Hui (100 mg/kg) group and low dose Peng-Hui (50 mg/kg) group. The T2DM control group was fed with only high-fat-diet. The normal control group was fed with common diet. The expression levels of IRS-1 in pancreas and skeletal muscle tissues were detected by immunohistochemistry method after 8-week treatment, and fasting blood glucose (FBG) and insulin levels were also detected in five groups. Results FBG lev-els were significantly lower in medium dose Peng-Hui group than those of T2DM control group and low dose Peng-Hui group (P＜0.05). There were no significant differences in insulin levels between high, medium and low dose Peng-Hui groups and T2DM control group (P>0.05). The expression levels of IRS-1 of pancreas and skeletal muscle tissues were significant-ly lower in high dose Peng-Hui group than that of T2DM control group (P＜0.05). Conclusion The expression of IRS-1 was decreased in high dose Peng-Hui group, but no effects on FBG and plasma insulin.
