Properties of Lipid Rafts from Cerebral Cortex Based on HPLC MS/MS Proteomics
10.3969/j.issn.0253-9896.2013.09.010
- VernacularTitle:液质联用蛋白组学技术分析脑组织脂筏样品的属性*
- Author:
Kun NIE
;
Xuezhu ZHANG
;
Lan ZHAO
;
Yujie JIA
;
Jingxian HAN
- Publication Type:Journal Article
- Keywords:
membrane lipids;
computational biology;
ultracentrifugation;
hypothalamus;
proteins;
genome;
lipid raft
- From:
Tianjin Medical Journal
2013;(9):881-883
- CountryChina
- Language:Chinese
-
Abstract:
Objective To verify the validity of the sucrose density gradient ultracentrifugation method for lipid rafts from cerebral cortex. Methods Extract lipid rafts from cerebral cortex in mouse were extracted by the sucrose density gradi-ent ultracentrifugation method. The properties of lipid rafts were detected by Western blotting method, double enzyme and light scattering methods. HPLC MS/MS proteomics and bioinformatics were used to locate proteins of lipid rafts in cells. Re-sults Lipid rafts from cerebral cortex were provided with the model properties of lipid rafts such as high light scattering and cholesterol and high expression of Flotillin-1. HPLC MS/MS proteomics identified total 647 proteins. Most of these pro-teins were from plasma membrane, endoplasmic reticulum, cytoskeleton and cytosol, however, there were 21% proteins among total 647 proteins were from nucleus, mitochondria and ribosomes. Conclusion The sucrose density gradient ultra-centrifugation method is a effective method to extract lipid rafts from cerebral cortex, however, the properties of mixture should be considered.
