The Expression and Significance of CRISP2 Gene in Different Types of Spermatogenic Failure
10.3969/j.issn.0253-9896.2013.11.004
- VernacularTitle:CRISP2基因在不同类型精子发生障碍中的表达及其意义
- Author:
Li JIANG
;
Mujun LI
;
Li QIN
- Publication Type:Journal Article
- Keywords:
spermatogenesis;
RNA;
RNA,messenger;
gene expression;
cysteine-rich secretory protein 2
- From:
Tianjin Medical Journal
2013;(11):1052-1054
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study sperm mRNA transcript expression of CRISP2 gene in the semen of patients with different types of spermatogenetic failure. Methods A total of 150 male infertility patients were divided into normal group, asthenozoospermia group and oligozoospermia group(n=50 for each group). Total RNA was extracted from sperm cells. Re-al-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) method was used to detect CRISP 2 gene mRNA levels in three groups. Results There was a significantly higher expression of CRISP2 in normal group (10.281 ± 2.173) than that of asthenozoospermia group (2.092±0.969, P＜0.05). There was no significant difference in the expression of CRISP2 between normal group and oligozoospermia group (9.420±2.794, P>0.05). The relative expression of CRISP2 was sig-nificantly lower in asthenozoospermia group than that of oligozoospermia group (P＜0.05). Conclusion The CRISP2 expres-sion was significantly different in patients with different types of spermatogenic failure. The reduced expression of CRISP 2 may lead to the decreased sperm motility.
