The Role of Expression of TGF-βand Smad Signaling Pathway andα-SMA in Patients with Diabetic Nephropathy
10.3969/j.issn.0253-9896.2013.11.008
- VernacularTitle:TGF-β、Smad信号通路蛋白及α-SMA在糖尿病肾病患者肾组织中的表达和意义
- Author:
Bolong FANG
;
Hongling HAN
;
Peng ZHANG
;
Jin CUI
- Publication Type:Journal Article
- Keywords:
diabetic nephropathies;
transforming growth factor beta;
Smad2 protein;
Smad3 protein;
actins;
signal transduction;
biopsy;
immunohistochemistry;
α-SMA
- From:
Tianjin Medical Journal
2013;(11):1067-1069
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of the pathogenesis of diabetic nephropathy (DN) by detecting the expressions of transforming growth factor (TGF)-β, Smad proteins andα-smooth muscle actin (α-SMA) in kidney biopsy of patients with diabetic nephropathy (DN). Methods Twenty-eight patients with DN who underwent renal biopsy were col-lected as DN group. Ten subjects without DN who underwent nephrectomy were taken as control group. The expressions of TGF-β1, TGF-βRⅠ,TGF-βRⅡ,Smad2/3 andα-SMA in renal tissues were detected by immunohistochemistry stain. Results (1)TGF-β1,TGF-β RⅠ,TGF-β RⅡ,and Smad2/3 were expressed in the glomeruli and tubules of both DN group and control group, while the expressions of TGF-βand Smad proteins were significantly higher in DN group than those in control group. At the early stage of DN, TGF-βand Smad proteins were significantly expressed even though there was no remarkable lesions observed by light microscopy. There was no correlation between increased expression and the progres-sion of DN. These proteins were not expressed after glomerulus and renal tubule fibrosis. (2) In control group,α-SMA was identified only in the vascular walls, glomerulus and renal tubules, while it was expressed in almost all parts of kidney in DN group. Conclusion TGF-β and Smad signals involved in the pathogenesis of DN, which may have similar pathogenesis with immune complex glomerulonephritis.
