Effects of Cyclic Tensile Strain on Proliferation of Human Osteoarthritis Chondrocytes
10.3969/j.issn.0253-9896.2013.11.010
- VernacularTitle:循环牵张应力对人骨关节炎软骨细胞增殖的影响
- Author:
Xiaolei SUN
;
Bin ZHAO
;
Xinlong MA
;
Xiulan LI
;
Jianxiong MA
;
Shuang LI
;
Qiang YANG
- Publication Type:Journal Article
- Keywords:
osteoarthritis;
cartilage,articular;
stress,mechanical;
cell proliferation;
cell cycle
- From:
Tianjin Medical Journal
2013;(11):1073-1075
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of cyclic tensile strain on the proliferation of cultured human osteoar-thritis chondrocytes. Methods The degenerated human cartilage came from a 66-year-old patient with osteoarthritis. The degree of degeneration was assessed by pathological diagnosis. Under the sterile condition, degenerated chondrocytes were cultured by enzymatic digestion. The toluidine blue staining, safranin O staining, and immunofluorescence staining of colla-genⅡwere used to identify the cultured cells. The third generation cells were seeded on the silicone rubber membrane carri-er, using the EF3200 mechanical tester equipped with BioDynamic bioreactor system, imposed a frequency of 0.25 Hz, stain 0, 5%, 10%and 15%load for 3 h. The proliferation activity was detected by flow cytometry. Results The proliferative in-dex (PI) increased with the magnitude value of cyclic tensile strain. The most significant increase of proliferation index was found in 10%group. Conclusion The proliferation of human osteoarthritis chondrocytes increases under some stress.
