The Application of DiversiLab System in Klebsiella pneumoniae Genotyping

VernacularTitle:应用DiversiLab系统对肺炎克雷伯菌进行同源性分析
Author: Jing LI ; Zhidong HU ; Bin TIAN ; Hairu XU
Publication Type:Journal Article
Keywords: Klebsiella pneumoniae; electrophoresis,gel,pulsed-field; bacterial typing techniques; DiversiLab system
From: Tianjin Medical Journal 2013;(11):1076-1078
CountryChina
Language:Chinese
Abstract: Objective To analyze Klebsiella pneumoniae by DiversiLab system, providing scientific evidence for the control of nosocomial infection. Methods Eight strains of non-duplicated clinical Klebsiella pneumoniae isolated from the surgical ward in a hospital in 2010 were typed by rep-PCR-based DiversiLab system. The results were compared with those of pulsed-field gel electrophoresis (PFGE). Results Antimicrobial susceptibility test showed that 7 clinical strains were the same sensitivity to 11 antimicrobial agents, except the strain K8-02. And these 7 clinical strains were all multi-drug re-sistant. The result of PFGE showed that 7 strains were the same pattern. The result of DiversiLab also showed that 7 strains were the same pattern, and the strain K8-02 was another pattern. Conclusion DiversiLab system is simple, quick, good re-peatability and accurate, which is a kind of standardization and automation system. DiversiLab system and PFGE method could get the same result.