The Expression of IGF2 in Mouse Embryonic Stem Cells to Differentiate into Islet-Like Cells
10.3969/j.issn.0253-9896.2013.11.021
- VernacularTitle:IGF2在小鼠胚胎干细胞定向分化为胰岛样细胞过程中的表达
- Author:
Feng LIU
;
Jiazhen TANG
;
Lingyan ZHU
;
Huaxia GAN
- Publication Type:Journal Article
- Keywords:
embryonic stem cells;
genomic imprinting;
insulin-like growth factorⅡ;
alleles;
cell differentiation
- From:
Tianjin Medical Journal
2013;(11):1111-1113
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of insulin-like growth factor-2 (IGF2) in the course of mouse embryon-ic stem cells induced to differentiate into islet-like cells. Methods Mouse ES cells were induced to differentiate into islet-like cells in vitro. The expression of islet specific markers was tested by RT-PCR and immunofluorescence assay. RT-PCR/RFLP was used to test the imprinted genes IGF2 parental expression in cells at different stages. Results Islet specific mark-ers were expressed in differentiated cells, such as insulin, glucagon and C-peptide. PCR-RFLP showed that imprinted genes IGF2 derived from embryonic stem cells were biallelic expression and loss of imprinting. Conclusion Gene imprinting sta-tus of IGF2 was changed in differentiated cells in vitro.
