Effect of microRNA-21 antisense oligonucleotide on collagen synthesis in the rat hepatic stellate cell s
10.3760/cma.j.issn.1008-1372.2015.03.005
- VernacularTitle:microRNA－21反义寡核苷酸对肝星状细胞胶原合成的影响
- Author:
Rongquan FU
;
Jiguang DING
;
Liang HONG
;
Qingfeng SUN
;
Jinguo WU
- Publication Type:Journal Article
- Keywords:
MicroRNAs;
Oligonucleotides,antisense/PD;
Hepatocytes/ME/DE;
Collagen/BI//DE
- From:
Journal of Chinese Physician
2015;(3):337-339
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of microRNA-21 (miR-21) antisense oligonucleoti-de on collagen synthesis in the rat hepatic satellite cells ( HSCs) .Methods Rat hepatic stellate cells were isolated and cultured; the miR-21 antisense oligonucleotide was transfected into HSCs with lipofectamine 2000;after incubation 48 h, the HSCs were collected.The expression of miR-21 was detected with reverse transcription polymerase chain reaction ( RT-PCR) , andα-smooth muscle actin (α-SMA) protein in HSCs with Western blot.The cell proliferation was assayed with methyl thiazolyl tetrazolium ( MTT) method.Re-sults Compared to scrambled control group, the expression of miR-21 was reduced by 76%( P <0.01), the proliferation activity of HSCs was reduced by(26 ±3)%( P <0.01), the expressions of type I and III collagen proteins were reduced by(61 ±7)%and (48 ±6)%( P <0.01).Conclusions The miR-21 an-tisense oligonucleotide could reduce significantly the expression of miR-21, and inhibit HSC proliferation and extracellular matrix production.
