PLCE1 suppresses apoptosis of lung adenocarcinoma cells via modulating p53 expression
10.3969/j.issn.1000-4718.2015.01.023
- VernacularTitle:PLCE1通过调控 p53抑制肺腺癌 A549细胞凋亡
- Author:
Yun LI
;
Xueping LUO
;
Jizong LIN
;
Shaohong HUANG
;
Jun AN
;
Jinyuan HE
;
Junhang ZHANG
- Publication Type:Journal Article
- Keywords:
Lung adenocarcinoma;
Phospholipase C epsilon 1;
p53;
Apoptosis;
Methylation;
Epigenomics
- From:
Chinese Journal of Pathophysiology
2015;(1):119-123
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the role of phospholipase C epsilon 1 ( PLCE1 ) in modulating the apoptotic mechanism in lung adenocarcinoma A 549 cells.METHODS:PLCE1 inhibitor U-73122 was used to suppress the expres-sion of PLCE1.The expression of PLCE1 and p53 in A549 cells was evaluated by quantitative real-time PCR and Western blotting.Apoptosis was assessed by flow cytometry .RESULTS:A549 cells expressed high level of PLCE1 and low level of p53.Inhibition of PLCE1 markedly increased the expression of p 53, and increased the apoptosis of A 549 cells.CON-CLUSION:PLCE1 suppresses apoptosis of A549 cells via inhibiting the expression of p53.
