Study of clinical application of HPV L1 capsid protein combined with HPV typing and TCT detec-tion in diagnosis and treatment of cervical lesion
10.3760/cma.j.issn.1008-1372.2015.03.013
- VernacularTitle:HPV L1壳蛋白联合 HPV 分型、TCT 检测在宫颈病变诊治中的临床应用研究
- Author:
Yaoqin SHEN
;
Juan LAI
;
Junxia HE
- Publication Type:Journal Article
- Keywords:
Papillomaviridae/ME;
Viral envelope proteins/ME;
Cytological techniques;
Uterine cervical diseases/ME/DI/TH
- From:
Journal of Chinese Physician
2015;(3):365-368
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of human papilloma virus L1 (HPV L1)capsid protein in cervical lesions and different human papillomavirus ( HPV) subtypes, and to guide clinical triage management and best individual treatment.Methods Retrospective analysis of 2012 January to 2014 Janu-ary in Jiaxing Hospital of Traditional Chinese Medicine gynecology clinic for HPV L1 protein combined with HPV type, and liquid-based cytology test ( TCT) of 176 patients data.Results The positive expression rate of HPV L1 protein with TCT examination in the negative for intraepithelial lesion or malignancy ( NILM) , atypical squamous cells of undetermined significance( ASCUS) , low-grade squamous intraepithe lial lesion ( LSIL) , atypical squamous cells not except high lesion ( ASC-H) , high-grade squamous intraep-ithelial lesion ( HSIL) , and squamous-cell carcinoma ( SCC) was 28.99%, 44.19%, 64.44%, 22.22%, 12.50%, and 0, respectively.No significant differences were found between the NILM and ASCUS groups ( P >0.05) .The positive expression rate of HPV L1 protein in LSIL group was the highest, and it was sta-tistically significantly different from ASC-H and HSIL groups (χ2 =3.88,5.50, P <0.05) , and 0 expres-sion in SCC group.It suggests that the positive expression rate of HPV L1 protein decreased with the severi-ty of cervical cytology lesions.The top five diseases with positive expression of HPV L1 protein were inflam-mation (44.76%), cervical intraepithelial neoplasia (CIN)Ⅰ(52.94%), CIN Ⅱ(23.81%), CIN Ⅲ(8.33%), and squamous cell carcinoma (0); no significant difference was found between inflammation group and CIN Ⅰ group ( P >0.05 ); and statistically significant difference was found between CIN Ⅰgroup and CINⅡ, CINⅢgroup (χ2 =4.53,5.56, P <0.05), and 0 expression in SCC group.It sug-gests that the positive expression rate of HPV L1 protein increased with the increased level of cervical le-sions.The positive expression rate of HPV L1 protein in top five frequency of HPV infection was 60.78%in multiple infection, 17.65%in type 16, 50%in type 52, 66.67%in type 58, and 12.5%in type 18, re-spectively.The expression rate of HPV L1 protein of Type 16 infection was significantly lower than that of type 52, type 58 infection and multi infection(χ2 =4.15, P <0.05, χ2 =7.88,15.44, P <0.01).The positive expression rate of HPV L1 protein of type 18 infection was lower than the other three, but significant differences occurred only compared to multiple infection(χ2 =4.70, P <0.05).The rest had no significant difference( P >0.05) .Conclusions Detection of HPV L1 protein is of clinical value to evaluate the risk of cervical lesions.HPV L1 protein combined with HPV type and TCT detection is helpful for traffic man-agement and personalized treatment, and benefit patients with cervical lesions.
