The protective role of mammalian target of rapamycin in the injury of podosytes induced by adriamycin

VernacularTitle:哺乳动物雷帕霉素靶蛋白在阿霉素诱导足细胞损伤中的作用
Author: Xiaoju ZHOU ; Yuning LI
Publication Type:Journal Article
Keywords: Podosytes; Adriamycin; mammalian target of rapamycin; Mouse
From: International Journal of Pediatrics 2014;41(6):644-648
CountryChina
Language:Chinese
Abstract: Objective To explore the role of mammalian target of rapamycin (mTOR) complex in the adriamycin induced the podecytes.Methods 1 μmol/L adriamycin (ADM) was used to induce mice podocytes for 24 hours and detected the apoptosis ratio,podosytes damage markers (Desmin) and the related expression of mTOR,then the role of mTOR was analysized in ADM induced podosytes.Results 1 umol/L ADM induced mice podosytes for 24 hours can make the podocytes damage.Compared with control group,podosytes activity declined(t =28.68,P ＜ 0.001),apoptosis rate increased both early (t =10.24,P =10.24) and late (t =4.26,P =4.26),and Desmin expression increased(t =6.16,P ＜0.001).mTOR expression decreased in ADM group.Compared with control group,both mTOR and phosphorylation mTOR expression decreased (t =3.57,P =3.57).mTOR downstream target molecular S6K1 and 4ebpl mRNA expression decreased(t =18.28,P ＜ 0.001).Conclusion ADM can make podosytes damage and the mechanism of ADM induced podosytes damage may be related to the decreasing mTOR and the low activity of mTOR.