The Analysis of HPV Genotypes and Related High Risk Factors in 459 Women of Annual Health Examination
10.3969/j.issn.0253-9896.2014.02.008
- VernacularTitle:459例女性健康体检者HPV基因型及高危因素分析
- Author:
Xiujie CHEN
;
Yanling LI
;
Pengpeng QU
- Publication Type:Journal Article
- Keywords:
papillomaviridae;
uterine cervical neoplasms;
carcinoma;
genotype;
risk factors;
polymerase chain reac-tion;
pyrosequencing
- From:
Tianjin Medical Journal
2014;(2):123-126
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the cervical human papillomavirus (HPV) infection, genotypes and related high risk factors in 459 women of annual health examination. Methods A total of 459 cervical cytology specimens were de-tected with nest PCR and pyrosequencing methods. Questionnaires were collected simultaneously and the risk factors of HPV infection were analyzed. Results The detection rate of HPV-positive samples was 17.9%. Seven kinds of high-risk genotypes of HPV were found. The detection of HPV 16 was the most common (9.8%), followed by HPV58 (7.0%) and HPV18 (5.2%). The detection rates of simplex infection, dual infections and multiple infections were 9.6%, 4.8%and 1.5%respectively. The risk factors for HPV infection included alcohol consumption,income <3 000 yuan/ month, sexual part-ners>1, frequency of sexual activity>4 times/month and cervical erosion (P<0.05). Conclusion HPV DNA genotyping can provide important reference for HPV screening at early time and the application of HPV vaccines, which also provide sig-nificant evidence for the prevention, diagnosis and treatment of cervical cancer at early stage for the high risk population.
