The expression of connective tissue growth factor and matrix metalloproteinase 9 in the ovary of rat models of polycystic ovarian syndrome
10.3969/j.issn.2095-4344.2015.27.027
- VernacularTitle:多囊卵巢综合征模型大鼠卵巢中结缔组织生长因子及基质金属蛋白酶9的表达
- Author:
Yanan SUN
- Publication Type:Journal Article
- Keywords:
Tissue Engineering;
Ovary;
Connective Tissue Growth Factor;
Matrix Metaloproteinase 9;
Extracelular Matrix
- From:
Chinese Journal of Tissue Engineering Research
2015;(27):4412-4416
- CountryChina
- Language:Chinese
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Abstract:
BACKGROUND:Several studies have demonstrated that the physiological process of the ovary, including folicle development, maturity, ovulation, corpus luteum formation and regression, is related to the formation of extracelular matrix. However, matrix metaloproteinase 9 and connective tissue growth factors had a close relationship with the formation of extracelular matrix. OBJECTIVE:To investigate the relationship between the expression of connective tissue growth factor and matrix metaloproteinase 9 and polycystic ovary syndrome. METHODS:The female SD rats with regular estrous cycle were randomly divided into control and model groups. The rats in the model group were intragastricaly with letrozole to establish the model of polycystic ovary syndrome, while the rats in the control group were intragastricaly with the same amount of carboxymethyl celulose. When the natural law of estrous cycle of rats in the model group did not exist and consecutive interval appeared, rat serum and ovarian tissues were colected. Rats in the control group were detected by colecting specimen in the interval. RESULTS AND CONCLUSION:Radioimmunoassay and immunohistochemical staining results showed that compared with the control group, the expression of serum luteinizing hormone, serum prolactin levels, connective tissue growth factor in preantral folicles theca cytoplasm, connective tissue growth factors in folicular basement membrane cytoplasm, connective tissue growth factors in albuginea and matrix metaloproteinase 9 in corpus luteum significantly increased (P < 0.05). The contents of folicle stimulating hormone and serum estradiol, and expression of matrix metaloproteinase 9 in folicular basement membrane significantly decreased (P < 0.05). The results indicate that connective tissue growth factors may be related to the excessive growth of smal folicles and ovulation disorders in polycystic ovary syndrome, while matrix metaloproteinase 9 may be related to ovulation disorders in polycystic ovary syndrome.
