Effect of meisoindigo on proliferation of leukemic cells regulated by bone marrow stromal cells
10.3760/cma.j.issn.1009-9921.2012.11.007
- VernacularTitle:甲异靛对白血病骨髓基质细胞调节白血病细胞增殖的影响
- Author:
Yi WANG
;
Xiaoyun LIAN
;
Ying GAO
;
Ding ZHANG
;
Hui WANG
;
Qishan WANG
- Publication Type:Journal Article
- Keywords:
Leukemia;
Meisoindigo;
Bone marrow stromal cells;
CXCR4
- From:
Journal of Leukemia & Lymphoma
2012;21(11):668-670
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of meisoindigo on co-culture leukemic cells and bone marrow stromal cells.Methods Bone marrow stromal cells were cultured using bone marrow mononuclear cells from patients with leukemia,and built the co-culture leukemic cells and bone marrow stromal cells.Trypan-blue-exclusion test detected the proliferation of leukemic cells.The expression levels of membrane CXCR4 on leukemic cells were assessed by flow cytometric analysis.Results Bone marrow stromal cell of leukemia could inhibit leukemic cells proliferation,leukemic cells could be promoted their growth when exposed to low concentration (5 μmol/L) of meisoindigo.Abnormal high expression of CXCR4 in leukemic cells,and meisoindigo could markedly inhibit the expression of CXCR4 in HL-60 and del leukemic cells.Expression of CXCR4 in leukemic cells can up-regulated by bone marrow stromal cells from patients with leukemia.Conclusion Meisoindigo can down regulate expression of CXCR4 in leukeimic cells to antileukemic cells.
