MgSO4 inhibited X-rays irradiation induced expression of γ-H2AX in HUVEC

VernacularTitle:硫酸镁抑制经辐射后人脐静脉血管内皮细胞γ-H2AX的表达
Author: Hualin XU ; Yang SUN ; Fengmei CUI ; Yu TU
Publication Type:Journal Article
Keywords: Magnesium sulfate; Human umbilical vein endothelial; X-rays; CCK-8; γ-H2AX
From: Chinese Journal of Radiological Medicine and Protection 2015;35(3):161-164
CountryChina
Language:Chinese
Abstract: Objective To investigate the influence of MgSO4 with different concentrations on cell survival and γ-H2AX expression in HUVEC irradiated with X-rays.Methods Cell proliferation rate was assayed by CCK-8,γ-H2AX foci formationwas observed with a laser confocal microscope,and γ-H2AX protein expression was detected by flow cytometer and Western blot assay.Results MgSO4 with a concentration of 1.25 mg/ml could improve the survival rate of IHUVEC treated with X-rays (t =-6.34,P ＜ 0.05).After irradiation,γ-H2AX loci approached to the highest level from 30 min to 1 h after radiation and then decreased.MgSO4 significantly reduced the foci formation at 0.5,1,2,6 and 12 h postirradiation (t =12.62,6.36,11.93,5.75,9.43,P ＜ 0.05).Both flow cytometry,and Western blot assays showed that MgSO4 inhibited γ-H2AX protein expression at 0.5,1 and 2 h post-irradiation (t =6.07,5.32,11.85,P ＜ 0.05).Conclusions MgSO4 could improve the survival rate and reduced γ-H2AX expression in HUVEC irradiated with X-ray.